This kit is optimized to detect human alpha-Synuclein (α-Synuclein) in cell and tissue lysate, and in human body fluids such as cerebrospinal fluid (CSF) and plasma. Wells are pre-coated with anti-α-Synuclein monoclonal antibodies, blocked, and stabilized for long-term storage. The amount of α-Synuclein is quantified using sandwich ELISA: rabbit polyclonal anti-α-Synuclein specific antibodies directly conjugated to horseradish peroxidase (HRP) are used to detect captured α-Synuclein.
This kit is optimized to detect rat alpha-Synuclein (α-Synuclein) in cell and tissue lysate, and in body fluids such as plasma. Wells are pre-coated with anti-α-Synuclein monoclonal antibodies, blocked, and stabilized for long-term storage. The amount of α-Synuclein is quantified using sandwich ELISA: rabbit polyclonal anti-α-Synuclein specific antibodies directly conjugated to horseradish peroxidase (HRP) are used to detect captured α-Synuclein.
SensoLyte® Transglutaminase Activity Assay Kit provides a convenient assay for the high throughput screening of TG modulators and inhibitors. TG catalyzes covalent bond formation between a free amine group of poly-D-lysine, which is coated on the plate surface, and γ-carboxamide group of glutamine in biotin-TVQQEL peptide substrate. The reaction immobilizes biotin-conjugated peptide to the plate surface. The amount of immobilized biotin is determined using streptavidin-horseradish peroxidase (HRP) andTMB substrate. Colorimetric signal generated by HRP and TMB substrate is proportional to the activity of TG in a sample. Assay is performed in a convenient 96-well microplate format.
SensoLyte® Red Transglutaminase Assay Kit, Fluorometric
1 KIT
Anaspec
SensoLyte® Transglutaminase Activity Assay Kit provides a convenient assay for the high throughput screening of TG modulators and inhibitors. TG catalyzes covalent bond formation between a free amine group of poly-D-lysine, which is coated on the plate surface, and γ-carboxamide group of glutamine in biotin-TVQQEL peptide substrate. The reaction immobilizes biotin-conjugated peptide to the plate surface. The amount of immobilized biotin is determined using streptavidin-horseradish peroxidase (HRP), 10-Acetyl-3,7-dihydroxyphenoxazine(ADHP), and hydrogen peroxide (H2O2). The non-fluorescent ADHP is oxidized to the strong fluorescent resorufin in the presence of H2O2 by peroxidases, such as HRP. The fluorescence intensity of resorufin is proportional to the activity of TG in a sample. Assay is performed in a convenient 96-well microplate format.
SensoLyte® Blue Glucocerebrosidase (GBA) Assay Kit *Fluorimetric*
1 KIT
Anaspec
The SensoLyte® Blue Glucocerebrosidase (GBA) Assay Kit detects GBA activity by using a most common fluorogenic substrate, MUGlc, for measurement of GBA activity. In presence of GBA the colorless substrate is hydrolyzed into 4-methylumbelliferone (4MU), and after addition of stop solution a bright blue fluorescence can be monitored at excitation/emission = 365nm/445nm nm.
SensoLyte® Red Glucocerebrosidase (GBA) Assay Kit *Fluorimetric*
1 KIT
Anaspec
The SensoLyte® Red Glucocerebrosidase (GBA) Assay Kit detects GBA activity by using a highly sensitive fluorogenic substrate. The GBA substrate provided in the kit releases the red fluorescent dye resorufin upon Glucocerebrosidase cleavage with absorption/emission maxima at 570 nm/610 nm. This assay kit can be used to detect enzyme activity in purified enzyme preparations and compound screening. The Red Glucocerebrosidase (GBA) Assay is one-step homogenous reaction, which does not require the additional step of dispensing stop solution after the incubation, thereby suitable for HTS. The long wavelength fluorescence resorufin is also less interfered by the autofluorescence of components in biological samples and test compounds.
This kit is optimized to detect human alpha-Synuclein (α-Synuclein) in cell and tissue lysate, and in human body fluids such as cerebrospinal fluid (CSF) and plasma. Wells are pre-coated with anti-α-Synuclein monoclonal antibodies, blocked, and stabilized for long-term storage. The amount of α-Synuclein is quantified using sandwich ELISA: rabbit polyclonal anti-α-Synuclein specific antibodies directly conjugated to horseradish peroxidase (HRP) are used to detect captured α-Synuclein.
This kit is optimized to detect rat alpha-Synuclein (α-Synuclein) in cell and tissue lysate, and in body fluids such as plasma. Wells are pre-coated with anti-α-Synuclein monoclonal antibodies, blocked, and stabilized for long-term storage. The amount of α-Synuclein is quantified using sandwich ELISA: rabbit polyclonal anti-α-Synuclein specific antibodies directly conjugated to horseradish peroxidase (HRP) are used to detect captured α-Synuclein.
SensoLyte® Transglutaminase Activity Assay Kit provides a convenient assay for the high throughput screening of TG modulators and inhibitors. TG catalyzes covalent bond formation between a free amine group of poly-D-lysine, which is coated on the plate surface, and γ-carboxamide group of glutamine in biotin-TVQQEL peptide substrate. The reaction immobilizes biotin-conjugated peptide to the plate surface. The amount of immobilized biotin is determined using streptavidin-horseradish peroxidase (HRP) andTMB substrate. Colorimetric signal generated by HRP and TMB substrate is proportional to the activity of TG in a sample. Assay is performed in a convenient 96-well microplate format.
SensoLyte® Red Transglutaminase Assay Kit, Fluorometric
Cat No.
ANA-AS-72245
규격
1 KIT
제조사
Anaspec
제품정보
SensoLyte® Transglutaminase Activity Assay Kit provides a convenient assay for the high throughput screening of TG modulators and inhibitors. TG catalyzes covalent bond formation between a free amine group of poly-D-lysine, which is coated on the plate surface, and γ-carboxamide group of glutamine in biotin-TVQQEL peptide substrate. The reaction immobilizes biotin-conjugated peptide to the plate surface. The amount of immobilized biotin is determined using streptavidin-horseradish peroxidase (HRP), 10-Acetyl-3,7-dihydroxyphenoxazine(ADHP), and hydrogen peroxide (H2O2). The non-fluorescent ADHP is oxidized to the strong fluorescent resorufin in the presence of H2O2 by peroxidases, such as HRP. The fluorescence intensity of resorufin is proportional to the activity of TG in a sample. Assay is performed in a convenient 96-well microplate format.
SensoLyte® Blue Glucocerebrosidase (GBA) Assay Kit *Fluorimetric*
Cat No.
AS-72258
규격
1 KIT
제조사
Anaspec
제품정보
The SensoLyte® Blue Glucocerebrosidase (GBA) Assay Kit detects GBA activity by using a most common fluorogenic substrate, MUGlc, for measurement of GBA activity. In presence of GBA the colorless substrate is hydrolyzed into 4-methylumbelliferone (4MU), and after addition of stop solution a bright blue fluorescence can be monitored at excitation/emission = 365nm/445nm nm.
SensoLyte® Red Glucocerebrosidase (GBA) Assay Kit *Fluorimetric*
Cat No.
AS-72259
규격
1 KIT
제조사
Anaspec
제품정보
The SensoLyte® Red Glucocerebrosidase (GBA) Assay Kit detects GBA activity by using a highly sensitive fluorogenic substrate. The GBA substrate provided in the kit releases the red fluorescent dye resorufin upon Glucocerebrosidase cleavage with absorption/emission maxima at 570 nm/610 nm. This assay kit can be used to detect enzyme activity in purified enzyme preparations and compound screening. The Red Glucocerebrosidase (GBA) Assay is one-step homogenous reaction, which does not require the additional step of dispensing stop solution after the incubation, thereby suitable for HTS. The long wavelength fluorescence resorufin is also less interfered by the autofluorescence of components in biological samples and test compounds.
EndoClear™ Human Recombinant alpha-Synuclein Protein
100 µg
Anaspec
Full-length Recombinant human alpha-synuclein (GenBank Accession # NP_000336) was expressed in E. coli., and purified from bacterial lysate using proprietary method. Endotoxin was further removed by proprietary technique. Molecular weight of the recombinant α-synuclein protein is 14.5 kDa.
EndoClear™ Mouse Recombinant alpha-Synuclein Protein
100 µg
Anaspec
Full-length Recombinant mouse α-synuclein (GenBank Accession # NP_001035916) was expressed in E. coli.,and purified from bacterial lysate using proprietary method. Endotoxin was further removed by proprietary technique. The molecular weight of the recombinant α-synuclein protein is 14,485 Da
EndoClear™ Rat Recombinant alpha-Synuclein Protein
100 µg
Anaspec
Full-length Recombinant rat α-synuclein (GenBank Accession # NP_062042) was expressed in E. coli., and purified from bacterial lysate using proprietary method. Endotoxin was further removed by proprietary technique. The molecular weight of the recombinant α-synuclein protein is 14,515 Da.
EndoClear™ Human Recombinant alpha-Synuclein Protein
Cat No.
ANA-AS-55555-100
규격
100 µg
제조사
Anaspec
제품정보
Full-length Recombinant human alpha-synuclein (GenBank Accession # NP_000336) was expressed in E. coli., and purified from bacterial lysate using proprietary method. Endotoxin was further removed by proprietary technique. Molecular weight of the recombinant α-synuclein protein is 14.5 kDa.
EndoClear™ Mouse Recombinant alpha-Synuclein Protein
Cat No.
ANA-AS-56082-100
규격
100 µg
제조사
Anaspec
제품정보
Full-length Recombinant mouse α-synuclein (GenBank Accession # NP_001035916) was expressed in E. coli.,and purified from bacterial lysate using proprietary method. Endotoxin was further removed by proprietary technique. The molecular weight of the recombinant α-synuclein protein is 14,485 Da
EndoClear™ Rat Recombinant alpha-Synuclein Protein
Cat No.
ANA-AS-56083-100
규격
100 µg
제조사
Anaspec
제품정보
Full-length Recombinant rat α-synuclein (GenBank Accession # NP_062042) was expressed in E. coli., and purified from bacterial lysate using proprietary method. Endotoxin was further removed by proprietary technique. The molecular weight of the recombinant α-synuclein protein is 14,515 Da.