The SensoLyte® 520 Calpain Activity Assay Kit is optimized for detecting calpain activity. This kit contains a novel internally quenched 5-FAM/QXL® 520 FRET substrate. Calpain protease cleaves the FRET substrate into two separate fragments resulting in the release of 5-FAM fluorescence which can be monitored at excitation/emission= 490/520 nm. Increase in fluorescence is proportional to the calpain activity. The assay can detect both calpain 1 (μ) and 2 (m) activities and is ideal for kinetic study of these enzymes. The long wavelength fluorescence of 5-FAM is less interfered by the autofluorescence of components in biological samples and test compounds. The assays are performed in a convenient 96-well microplate format. Kit size: 100 assays
The SensoLyte® Rh110 Matriptase Assay Kit provides a convenient assay for screening of enzyme inhibitors and activators or for continuous assay of enzyme activity using a fluorogenic substrate. Upon matriptase cleavage, this substrate generates the Rh110 (Rhodamine 110) fluorophore which has a bright green fluorescence and can be detected at excitation/emission=496 nm/520 nm. The longer wavelength spectra and higher extinction coefficient of Rh110 provide greater sensitivity and less interference from other reaction components. This assay can detect as low as 0.15 ng/mL active Matriptase.
The SensoLyte® Caspase Profiling Kit contains a series of AMC-based peptide substrates as fluorogenic indicators for assaying caspase protease activities. The kit contains a well-designed plate in which a series of AMC-based caspase substrates are coated with both positive and negative controls. It provides the best solution for profiling caspases or caspase inhibitors. *384-well plate format is available on custom basis. Kit Size: 2 plates
The SensoLyte® Homogeneous AMC Capase-3/7 Assay Kit uses Ac-DEVD-AMC as the fluorogenic indicator for assaying caspase-3/7 activities. Upon caspase-3/7 cleavage, Ac-DEVD-AMC generates the AMC fluorophore which has bright blue fluorescence and can be detected at Ex/Em=354 nm/442 nm. A bi-function assay buffer in this kit is designed to lyze the cells and measure the enzyme activity at the same time. Thus, this kit can measure caspase-3/7 activity in cell culture directly in a 96-well or 384-well plate without a time-consuming cell extraction step. In case the cells are cultured in larger plates or flasks, a lysis buffer and protocol for cell lysate preparation are also conveniently included in the kit. The kit is suitable for high throughput screening of apoptosis inducers and inhibitors.
SensoLyte® 520 Cathepsin D Assay Kit *Fluorimetric*
Anaspec
The SensoLyte® 520 Cathepsin D Activity Assay Kit uses a QXL® 520/HiLyte™ Fluor 488 labeled FRET peptide substrate for measurement of enzyme activity. In the intact FRET peptide, the fluorescence of HiLyte™ Fluor 488 is quenched by QXL® 520. Upon cleavage of the FRET peptide by the active enzyme, the increase of fluorescence can be continuously monitored at excitation/emission = 490 nm/520 nm. The fluorescent signal from HiLyte™ Fluor 488 is stable at low pH, which is optimal for cathepsin activity. The kit can be used to detect the activity of Cathepsin D enzyme in biological samples and purified enzyme preparations. Kit size: 100 assays (96-well plate)
SensoLyte® 520 Cathepsin E Assay Kit *Fluorimetric*
Anaspec
The SensoLyte® 520 Cathepsin E Activity Assay Kit is a homogeneous assay that can be used to detect the enzyme activity in biological samples or in purified enzyme preparations. The unique long wavelength FRET substrate employed in the assay was designed to reduce the cross reactivity of Cathepsin D. A QXL® 520/HiLyte™ Fluor 488 pair is used for optimal quenching of the intact substrate. When active Cathepsin E cleaves the FRET substrate, it results in an increase of HiLyte™ Fluor 488 fluorescence, monitored at excitation/emission = 490 nm/520 nm. The fluorescent signal from HiLyte™ Fluor 488 is stable at low pH, an optimal pH for cathepsin activity. This assay can detect as low as 2 ng/mL active Cathepsin E. Kit size: 100 assays (96-well plate)
Sensolyte® Cell Viability and Proliferation Assay Kit
1 kit
Anaspec
The SensoLyte® Cell Viability and Proliferation Assay Kit provides researchers with a convenient one-solution and one-step assay to count living cells in a culture and continuously monitor cell proliferation over time by measuring cytoplasmic LDH activity. In this kit, resazurin is used as a sensitive fluorogenic indicator. Resazurin is converted to the strongly fluorescent resorufin (Ex/Em=560nm/590 nm) by cytoplasmic LDH. The kit can detect as few as 48 living cells with a linear range up to 5X104 cells (R2=0.99). This kit can also be used for high throughput screening of cell proliferation or measuring cytotoxicity effect of a variety of compounds. 384-well or 1536-well format can be used with minor modifications. Kit Size: 2000 assays This assay is also available in a larger size (10,000 assays)
The SensoLyte® Cell Cytotoxicity Assay Kit uses resazurin as a sensitive fluorogenic indicator (Ex/Em=560 nm/590 nm upon conversion) to measure LDH activity. The assay can be performed in a mixed population of damaged and viable cells, but it only measures the LDH released from damaged cells. The cytoplasmic LDH in living cells produces little signals under assay condition. There is no need for extra steps to separate living cells and supernatant. The fluorescent signal is proportional to the number of damaged cells (up to 2.5X104 cell, r2>0.95) with the detection limit reaching 100 dead cells. The kit is suitable for high throughput screening of cytotoxicity of a variety of compounds. 384-well or 1536-well format can be used with minor modifications. Kit Size: 500 assays
The SensoLyte® Calcein Cell Viability Assay Kit provides a convenient tool to detect live cells. This kit can be used in proliferation, cytotoxicity, invasion, adhesion, migration and other cell-based assays. Kit Size: 1000 assays
The FRET substrate included in SensoLyte® 520 Meprin α Activity Assay Kit was designed to detect Meprin α activity and reduce the cross reactivity with Meprin β, ADAM10, α -secretase, BACE-2, ECEs and TACE. This assay kit can be used to detect enzyme activity in purified enzyme preparations and compound screening. Active Meprin α cleaves the FRET substrate, resulting in an increase of 5-FAM fluorescence, monitored at excitation/emission = 490 nm/520 nm. The long wavelength fluorescence of 5-FAM is also less interfered by the autofluorescence of components in biological samples and test compounds. This assay can detect as low as 0.1 ng/mL active Meprin α. Kit size: 100 assays (96-well plate)
The SensoLyte® 520 Meprin β Activity Assay Kit can be used to detect enzyme activity in purified enzyme preparations and compound screening. The unique FRET substrate was derived from APP sequence designed to reduce the cross reactivity with Meprin α, ADAM10, α-secretase, BACE-2, IDE and ECEs, Neprilysin and TACE. Active Meprin β cleaves the FRET substrate resulting in an increase of 5-FAM fluorescence, that can be monitored at excitation/emission = 490 nm/520 nm. The long wavelength fluorescence of 5-FAM is also less interfered by the autofluorescence of components in biological samples and test compounds. This assay can detect as low as 0.048 ng/mL active Meprin β. Kit size: 100 assays (96-well plate)
The SensoLyte® 520 Calpain Activity Assay Kit is optimized for detecting calpain activity. This kit contains a novel internally quenched 5-FAM/QXL® 520 FRET substrate. Calpain protease cleaves the FRET substrate into two separate fragments resulting in the release of 5-FAM fluorescence which can be monitored at excitation/emission= 490/520 nm. Increase in fluorescence is proportional to the calpain activity. The assay can detect both calpain 1 (μ) and 2 (m) activities and is ideal for kinetic study of these enzymes. The long wavelength fluorescence of 5-FAM is less interfered by the autofluorescence of components in biological samples and test compounds. The assays are performed in a convenient 96-well microplate format. Kit size: 100 assays
The SensoLyte® Rh110 Matriptase Assay Kit provides a convenient assay for screening of enzyme inhibitors and activators or for continuous assay of enzyme activity using a fluorogenic substrate. Upon matriptase cleavage, this substrate generates the Rh110 (Rhodamine 110) fluorophore which has a bright green fluorescence and can be detected at excitation/emission=496 nm/520 nm. The longer wavelength spectra and higher extinction coefficient of Rh110 provide greater sensitivity and less interference from other reaction components. This assay can detect as low as 0.15 ng/mL active Matriptase.
The SensoLyte® Caspase Profiling Kit contains a series of AMC-based peptide substrates as fluorogenic indicators for assaying caspase protease activities. The kit contains a well-designed plate in which a series of AMC-based caspase substrates are coated with both positive and negative controls. It provides the best solution for profiling caspases or caspase inhibitors. *384-well plate format is available on custom basis. Kit Size: 2 plates
The SensoLyte® Homogeneous AMC Capase-3/7 Assay Kit uses Ac-DEVD-AMC as the fluorogenic indicator for assaying caspase-3/7 activities. Upon caspase-3/7 cleavage, Ac-DEVD-AMC generates the AMC fluorophore which has bright blue fluorescence and can be detected at Ex/Em=354 nm/442 nm. A bi-function assay buffer in this kit is designed to lyze the cells and measure the enzyme activity at the same time. Thus, this kit can measure caspase-3/7 activity in cell culture directly in a 96-well or 384-well plate without a time-consuming cell extraction step. In case the cells are cultured in larger plates or flasks, a lysis buffer and protocol for cell lysate preparation are also conveniently included in the kit. The kit is suitable for high throughput screening of apoptosis inducers and inhibitors.
SensoLyte® 520 Cathepsin D Assay Kit *Fluorimetric*
Cat No.
ANA-AS-72170
규격
제조사
Anaspec
제품정보
The SensoLyte® 520 Cathepsin D Activity Assay Kit uses a QXL® 520/HiLyte™ Fluor 488 labeled FRET peptide substrate for measurement of enzyme activity. In the intact FRET peptide, the fluorescence of HiLyte™ Fluor 488 is quenched by QXL® 520. Upon cleavage of the FRET peptide by the active enzyme, the increase of fluorescence can be continuously monitored at excitation/emission = 490 nm/520 nm. The fluorescent signal from HiLyte™ Fluor 488 is stable at low pH, which is optimal for cathepsin activity. The kit can be used to detect the activity of Cathepsin D enzyme in biological samples and purified enzyme preparations. Kit size: 100 assays (96-well plate)
SensoLyte® 520 Cathepsin E Assay Kit *Fluorimetric*
Cat No.
ANA-AS-72222
규격
제조사
Anaspec
제품정보
The SensoLyte® 520 Cathepsin E Activity Assay Kit is a homogeneous assay that can be used to detect the enzyme activity in biological samples or in purified enzyme preparations. The unique long wavelength FRET substrate employed in the assay was designed to reduce the cross reactivity of Cathepsin D. A QXL® 520/HiLyte™ Fluor 488 pair is used for optimal quenching of the intact substrate. When active Cathepsin E cleaves the FRET substrate, it results in an increase of HiLyte™ Fluor 488 fluorescence, monitored at excitation/emission = 490 nm/520 nm. The fluorescent signal from HiLyte™ Fluor 488 is stable at low pH, an optimal pH for cathepsin activity. This assay can detect as low as 2 ng/mL active Cathepsin E. Kit size: 100 assays (96-well plate)
Sensolyte® Cell Viability and Proliferation Assay Kit
Cat No.
ANA-AS-71300
규격
1 kit
제조사
Anaspec
제품정보
The SensoLyte® Cell Viability and Proliferation Assay Kit provides researchers with a convenient one-solution and one-step assay to count living cells in a culture and continuously monitor cell proliferation over time by measuring cytoplasmic LDH activity. In this kit, resazurin is used as a sensitive fluorogenic indicator. Resazurin is converted to the strongly fluorescent resorufin (Ex/Em=560nm/590 nm) by cytoplasmic LDH. The kit can detect as few as 48 living cells with a linear range up to 5X104 cells (R2=0.99). This kit can also be used for high throughput screening of cell proliferation or measuring cytotoxicity effect of a variety of compounds. 384-well or 1536-well format can be used with minor modifications. Kit Size: 2000 assays This assay is also available in a larger size (10,000 assays)
The SensoLyte® Cell Cytotoxicity Assay Kit uses resazurin as a sensitive fluorogenic indicator (Ex/Em=560 nm/590 nm upon conversion) to measure LDH activity. The assay can be performed in a mixed population of damaged and viable cells, but it only measures the LDH released from damaged cells. The cytoplasmic LDH in living cells produces little signals under assay condition. There is no need for extra steps to separate living cells and supernatant. The fluorescent signal is proportional to the number of damaged cells (up to 2.5X104 cell, r2>0.95) with the detection limit reaching 100 dead cells. The kit is suitable for high throughput screening of cytotoxicity of a variety of compounds. 384-well or 1536-well format can be used with minor modifications. Kit Size: 500 assays
The SensoLyte® Calcein Cell Viability Assay Kit provides a convenient tool to detect live cells. This kit can be used in proliferation, cytotoxicity, invasion, adhesion, migration and other cell-based assays. Kit Size: 1000 assays
The FRET substrate included in SensoLyte® 520 Meprin α Activity Assay Kit was designed to detect Meprin α activity and reduce the cross reactivity with Meprin β, ADAM10, α -secretase, BACE-2, ECEs and TACE. This assay kit can be used to detect enzyme activity in purified enzyme preparations and compound screening. Active Meprin α cleaves the FRET substrate, resulting in an increase of 5-FAM fluorescence, monitored at excitation/emission = 490 nm/520 nm. The long wavelength fluorescence of 5-FAM is also less interfered by the autofluorescence of components in biological samples and test compounds. This assay can detect as low as 0.1 ng/mL active Meprin α. Kit size: 100 assays (96-well plate)
The SensoLyte® 520 Meprin β Activity Assay Kit can be used to detect enzyme activity in purified enzyme preparations and compound screening. The unique FRET substrate was derived from APP sequence designed to reduce the cross reactivity with Meprin α, ADAM10, α-secretase, BACE-2, IDE and ECEs, Neprilysin and TACE. Active Meprin β cleaves the FRET substrate resulting in an increase of 5-FAM fluorescence, that can be monitored at excitation/emission = 490 nm/520 nm. The long wavelength fluorescence of 5-FAM is also less interfered by the autofluorescence of components in biological samples and test compounds. This assay can detect as low as 0.048 ng/mL active Meprin β. Kit size: 100 assays (96-well plate)
Caspase1 Inhibitor II is also known as IL-1β Converting Enzyme (ICE) Inhibitor; Biotin-YVAD-CMK rescues neuronal cells from cell death in response to oxidative stress and oxygen/glucose deprivation.
AMC (7-amino-4-methylcoumarin)-derived caspase substrates are widely used for the fluorimetric detection of various caspase activities. Cleavage of AMC peptides by caspases generates strongly fluorescent AMC that is monitored fluorimetrically at 440-460 nm with excitation of 340-350 nm.
This is amino acids 25 to 33 fragment of human melanoma antigen gp100. This H-2Db restricted epitope is recognized by T cells. The gp100-specific, H-2Db-restricted, CD8+ T cells are capable of recognizing B16 melanoma but not normal melanocytes. This peptide was used as an immunogen in multiple cancer immunotherapy studies.
c-Myc, the product of the c-myc proto-oncogene, is a helix-loop-helix leucine zipper phosphoprotein that regulates gene transcription in cell proliferation, cell differentiation and apoptosis. This peptide is a human c-myc epitope.
This is amino acids 17 to 26 fragment of p53, fluorescent labeled through an LC spacer. This peptide is the Mdm-2 binding domain of p53 known also as p53N. This sequence contains all of the residues that come into contact with the binding domain of Mdm-2. The tumor suppressor protein p53 is important in maintaining genome stability and in preventing cancer development, FITC (Abs/Em=493 nm/517 nm)
Human PD-L1 inhibitor I is a peptide-based molecule. It binds to human PD-1 and inhibits PD-1/PD-L1 binding. This peptide, PDL-1 inhibitor I has anchor residues, WDY that influence binding of hPD-L1 to hPD-1. Developing inhibitors specifically blocking the PD-1/PD-L1 pathway has become a popular approach toward cancer treatment.
Human PD-L1 inhibitor II is a peptide-based molecule. It binds to human PD-1 and inhibits PD-1/PD-L1 binding. This peptide, PDL-1 inhibitor I has anchor residues, WDY that influence binding of hPD-L1 to hPD-1. Developing inhibitors specifically blocking the PD-1/PD-L1 pathway has become a popular approach toward cancer treatment.
Human PD-L1 inhibitor III is a peptide-based molecule. It binds to human PD-1 and inhibits PD-1/PD-L1 binding. This peptide, hPDL-1 inhibitor III has anchor residues (underlined), TEKDYRHGNIRMKLAYDL that influence binding of hPD-L1 to hPD-1. Developing inhibitors specifically blocking the PD-1/PD-L1 pathway has become a popular approach toward cancer treatment.
Human PD-L1 inhibitor IV is a peptide-based molecule. It binds to human PD-1 and inhibits PD-1/PD-L1 binding. This peptide, hPDL-1 inhibitor IV has anchor residues (underlined), GNWDYNSQRAQLYNQ that influence binding of hPD-L1 to hPD-1. Developing inhibitors specifically blocking the PD-1/PD-L1 pathway has become a popular approach toward cancer treatment.
Human PD-L1 inhibitor V is a peptide-based molecule. It binds to human PD-1 and inhibits PD-1/PD-L1 binding. This peptide, hPDL-1 inhibitor V has anchor residues (underlined), LDYVNRRKMYQ that influence binding of hPD-L1 to hPD-1. Developing inhibitors specifically blocking the PD-1/PD-L1 pathway has become a popular approach toward cancer treatment.
Caspase1 Inhibitor II is also known as IL-1β Converting Enzyme (ICE) Inhibitor; Biotin-YVAD-CMK rescues neuronal cells from cell death in response to oxidative stress and oxygen/glucose deprivation.
AMC (7-amino-4-methylcoumarin)-derived caspase substrates are widely used for the fluorimetric detection of various caspase activities. Cleavage of AMC peptides by caspases generates strongly fluorescent AMC that is monitored fluorimetrically at 440-460 nm with excitation of 340-350 nm.
This is amino acids 25 to 33 fragment of human melanoma antigen gp100. This H-2Db restricted epitope is recognized by T cells. The gp100-specific, H-2Db-restricted, CD8+ T cells are capable of recognizing B16 melanoma but not normal melanocytes. This peptide was used as an immunogen in multiple cancer immunotherapy studies.
c-Myc, the product of the c-myc proto-oncogene, is a helix-loop-helix leucine zipper phosphoprotein that regulates gene transcription in cell proliferation, cell differentiation and apoptosis. This peptide is a human c-myc epitope.
This is amino acids 17 to 26 fragment of p53, fluorescent labeled through an LC spacer. This peptide is the Mdm-2 binding domain of p53 known also as p53N. This sequence contains all of the residues that come into contact with the binding domain of Mdm-2. The tumor suppressor protein p53 is important in maintaining genome stability and in preventing cancer development, FITC (Abs/Em=493 nm/517 nm)
Human PD-L1 inhibitor I is a peptide-based molecule. It binds to human PD-1 and inhibits PD-1/PD-L1 binding. This peptide, PDL-1 inhibitor I has anchor residues, WDY that influence binding of hPD-L1 to hPD-1. Developing inhibitors specifically blocking the PD-1/PD-L1 pathway has become a popular approach toward cancer treatment.
Human PD-L1 inhibitor II is a peptide-based molecule. It binds to human PD-1 and inhibits PD-1/PD-L1 binding. This peptide, PDL-1 inhibitor I has anchor residues, WDY that influence binding of hPD-L1 to hPD-1. Developing inhibitors specifically blocking the PD-1/PD-L1 pathway has become a popular approach toward cancer treatment.
Human PD-L1 inhibitor III is a peptide-based molecule. It binds to human PD-1 and inhibits PD-1/PD-L1 binding. This peptide, hPDL-1 inhibitor III has anchor residues (underlined), TEKDYRHGNIRMKLAYDL that influence binding of hPD-L1 to hPD-1. Developing inhibitors specifically blocking the PD-1/PD-L1 pathway has become a popular approach toward cancer treatment.
Human PD-L1 inhibitor IV is a peptide-based molecule. It binds to human PD-1 and inhibits PD-1/PD-L1 binding. This peptide, hPDL-1 inhibitor IV has anchor residues (underlined), GNWDYNSQRAQLYNQ that influence binding of hPD-L1 to hPD-1. Developing inhibitors specifically blocking the PD-1/PD-L1 pathway has become a popular approach toward cancer treatment.
Human PD-L1 inhibitor V is a peptide-based molecule. It binds to human PD-1 and inhibits PD-1/PD-L1 binding. This peptide, hPDL-1 inhibitor V has anchor residues (underlined), LDYVNRRKMYQ that influence binding of hPD-L1 to hPD-1. Developing inhibitors specifically blocking the PD-1/PD-L1 pathway has become a popular approach toward cancer treatment.