제품특징
- Formaldehyde-fixed cell samples에서 open chromatin 연구가 가능한 ATAC-seq kit
- Sample preparation, tagmentation, library preparation 과정을 통하여 next-gen sequencing-ready libraries를 얻을 수 있음
- 50,000~100,000 cells만으로도 실험 가능
- 간단하고 빠른 실험이 가능하도록 optimized된 protocol과 시약


Figure 1. Fixed Cell ATAC-Seq Compared to ATAC-Seq on Native Frozen Cell ATAC-Seq

Figure 2. Fixed Cell Titration ATAC-Seq Results
제품사양
Hypotonic Buffer |
store at RT |
Hypotonic Quench |
store at 4°C |
Releasing Buffer |
store at RT |
Proteinase K |
store at -20°C |
RNase A |
store at -20°C |
Protease Inhibitor Cockatil |
store at -20°C |
Assembled Transposomes |
store at -20°C |
2X Tagmentation Buffer |
store at -20°C |
10X PBS |
store at RT |
10% Tween 20 |
store at RT |
1% Digitonin |
store at -20°C |
0.5 M EDTA |
store at RT |
DNA Purification Columns SF |
store at RT |
DNA Purification Binding Buffer |
store at RT |
DNA Purification Wash Buffer |
store at RT |
DNA Purification Elution Buffer |
store at RT |
3 M Sodium Acetate |
store at RT |
10 mM dNTPs |
store at -20°C |
5X Q5 Buffer |
store at -20°C |
Q5 High-Fidelity DNA Polymerase |
store at -20°C |
i7 Indexed Primer 1 |
store at -20°C |
i7 Indexed Primer 2 |
store at -20°C |
i7 Indexed Primer 3 |
store at -20°C |
i7 Indexed Primer 4 |
store at -20°C |
i5 Indexed Primer 1 |
store at -20°C |
i5 Indexed Primer 2 |
store at -20°C |
i5 Indexed Primer 3 |
store at -20°C |
i5 Indexed Primer 4 |
store at -20°C |
SPRI Beads |
store at 4°C |