제품특징

Advantages

- 세포 내 및 세포 간 신호 전달, 자가 증식 줄기 세포, 세포 안팎으로 기능적 수송을 포함하여 intestinal epithelium의 많은 주요 특성을 요약하는 편리한 in vitro 시스템

- 일관된 결과를 위한 complete medium formulation

- 1주일 만에 intestinal organoid 생성 가능

- 사용하기 쉬운 형식과 최적화된 프로토콜

10-14일의 장 오가노이드 생성 과정

Figure 1. Primary Organoids Grown in IntestiCult™ Organoid Growth Medium (Human) are Fully Mature After 10-14 Days in Culture

Primary organoids were cultured from human colonic biopsy samples and grown in IntestiCult Organoid Growth Medium (Human). Organoids were imaged after (A) two days, (B) six days, (C) eight days and (D) ten days growth.

Organoid 형성 후 분석: epithelial layer marker(EPICAM) 발현 확인

Figure 2. Organoids Grown in IntestiCult™ Organoid Growth Medium (Human) Display Markers of Human Intestinal Epithelial Cells

Immunofluorescence of organoids grown in IntestiCult™ Organoid Growth Medium (Human) showing colocalization of (A) DAPI, (B) EPCAM and (C) Ki67. (D) A merged image shows the position of actively proliferating (Ki67+) intestinal stem cells within the epithelial layer (EPCAM+).

다양한 실험에 적용 가능

Figure 3. Forskolin-Induced Swelling of Organoids Grown in IntestiCult™ Organoid Growth Medium (Human)

Organoids were treated with (A) 5 μM Forskolin or (B) DMSO and organoid area was measured at 0 minutes and 120 minutes. (C)Forskolin-treated organoids increased in size 33.5 ± 3.8% compared to 7.5 ± 0.8% for DMSO-treated organoids.

Publication

- ACS infectious diseases 2020 may

Fucose-Galactose Polymers Inhibit Cholera Toxin Binding to Fucosylated Structures and Galactose-Dependent Intoxication of Human Enteroids.

J. Cervin et al.

- Nature communications 2020 may

TNFAIP8 controls murine intestinal stem cell homeostasis and regeneration by regulating microbiome-induced Akt signaling.

J. R. Goldsmith et al.

- Toxicology in vitro : an international journal published in association with BIBRA 2020 jul

Human ileal organoid model recapitulates clinical incidence of diarrhea associated with small molecule drugs.

D. G. Belair et al.

- The Journal of clinical investigation 2020 jan

Apelin directs endothelial cell differentiation and vascular repair following immune-mediated injury.

A. G. Masoud et al.

- Journal of animal science 2020 feb

Evaluation of swine enteroids as in vitro models for Lawsonia intracellularis infection1,2.

T. P. Resende et al.

관련 논문 더 보기: Search results for: '06010' (stemcell.com)

주문정보

	Cat No.	품명	규격	제조사	제품정보	수량	견적문의
	ST06010	IntestiCult™ Organoid Growth Medium (Human)	1 kit	StemCell Technologies	Cell culture medium for establishment and maintenance of human intestinal organoids		카트담기 견적문의

	전체선택
	IntestiCult™ Organoid Growth Medium (Human) Cat No. ST06010 규격 1 kit 제조사 StemCell Technologies 제품정보 Cell culture medium for establishment and maintenance of human intestinal organoids 수량 카트담기 견적문의

관련제품

	Cat No.	품명	규격	제조사	제품정보	수량	견적문의
	ST100-1061	CryoStor® CS10	100mL	STEMCELL Technologies	Animal component-free, defined cryopreservation medium with 10% DMSO		카트담기 견적문의
	ST100-0340	IntestiCult™-SF Organoid Growth Medium (Human)	100mL	StemCell Technologies	Serum-free and conditioned medium-free cell culture medium for establishment and maintenance of human intestinal organoids		카트담기 견적문의
	ST100-0214	IntestiCult™ Organoid Differentiation Medium (Human)	1 kit	StemCell Technologies	Cell culture medium for further differentiation of human intestinal organoids in 3D, or as monolayers/air-liquid interface cultures		카트담기 견적문의
	ST06005	IntestiCult™ Organoid Growth Medium (Mouse)	1 kit	StemCell Technologies	Cell culture medium for establishment and maintenance of mouse intestinal organoids		카트담기 견적문의

	전체선택
	CryoStor® CS10 Cat No. ST100-1061 규격 100mL 제조사 STEMCELL Technologies 제품정보 Animal component-free, defined cryopreservation medium with 10% DMSO 수량 카트담기 견적문의
	IntestiCult™-SF Organoid Growth Medium (Human) Cat No. ST100-0340 규격 100mL 제조사 StemCell Technologies 제품정보 Serum-free and conditioned medium-free cell culture medium for establishment and maintenance of human intestinal organoids 수량 카트담기 견적문의
	IntestiCult™ Organoid Differentiation Medium (Human) Cat No. ST100-0214 규격 1 kit 제조사 StemCell Technologies 제품정보 Cell culture medium for further differentiation of human intestinal organoids in 3D, or as monolayers/air-liquid interface cultures 수량 카트담기 견적문의
	IntestiCult™ Organoid Growth Medium (Mouse) Cat No. ST06005 규격 1 kit 제조사 StemCell Technologies 제품정보 Cell culture medium for establishment and maintenance of mouse intestinal organoids 수량 카트담기 견적문의

최근 본 제품