제품특징

Principle 

-  FRET peptide 형광 측정 방식 사용으로 고감도 검출 가능

  : HTS에 이상적인 Homogeneous assay format

High Sensitivity

- 520 Enterokinase Activity Assay Kit: 소량의 1.25ng/mL enterokinase activity 확인 가능

- IDE Activity Assay Kit: 소량의 0.8ng/mL IDE activity 확인 가능

- Neprilysin Activity Assay Kit: 소량의 0.78ng/mL Neprilysin activity 확인 가능

- Rh110 Enterokinase Activity Assay Kit: 소량의 0.31ng/mL enterokinase activity 확인 가능

Easy to Use 

- One-step & Mix-and-Read format으로 간편한 실험 방식

- 넉넉한 Reagent 제공: 100 assay

- Drug Discovery 어플리케이션 용 HTS와 호환

Assay Kit Case Study

- Inhibition of DPPIV activity measured with SensoLyte®Rh110 DPPIV Assay Kit

DPPIV also known as CD26 is a widely distributed serine protease. Its activity is critical for inactivation of GLP-1, a potent antihyperglycemic hormone, inducing glucose-dependent stimulation of insulin secretion. DPPIV inhibitors provide a potent treatment for type 2 diabetes prolonging the activity of GLP-1 and improving insulin secretion and blood glucose control regulation 1-2. The SensoLyte® Rh110 DPPIV Assay Kit provides a convenient method for high throughput screening of DPPIV inhibitors and for continuous assay of DPPIV activity utilizing a fluorogenic peptide. Upon enzyme cleavage, the peptide releases the Rh110 fluorophore with bright green fluorescence that can be detected at Ex/Em=490/520 nm. The longer-wavelength spectra and higher extinction coefficient of the Rh110 provide greater sensitivity and less interference from reaction components. 

Citations 

1. Zhu, L. et al, J. Biol. Chem 278, 22418 (2003).

2. Mentlein, R. Regulatory Peptides 85, 9 (1998).

주문정보

	Cat No.	품명	규격	제조사	제품정보	수량	견적문의
	AS-72209	SensoLyte ® 520 Enterokinase Activity Assay Kit *Fluorimetric*	1 kit	Anaspec	The SensoLyte® 520 Enterokinase Assay Kit is optimized for detecting activity of enterokinase. This kit employs a novel internally quenched 5-FAM/QXL FRET substrate for enterokinase. Enterokinase cleaves the FRET substrate into two separate fragments resulting in release of 5-FAM fluorescence which can be monitored at excitation/emission= 490/520 nm. The long wavelength fluorescence of 5-FAM is less interfered by the autofluorescence of components in biological samples and test compounds. The assay can detect as low as 1.25 ng/mL of active enterokinase. Kit size: 100 assays		카트담기 견적문의
	AS-72231	SensoLyte ® 520 IDE Activity Assay Kit *Fluorimetric*	1 kit	Anaspec	The SensoLyte® 520 IDE Activity Assay Kit can be used to detect enzyme activity in biological samples or in purified enzyme preparations. The unique FRET substrate was derived from an APP sequence designed to reduce cross reactivity with Neprilysin, ADAM10, TACE, BACE-1, and BACE-2. When active IDE cleaves the FRET substrate, it results in an increase of 5-FAM fluorescence, which is monitored at excitation/emission = 490 nm/520 nm. The long wavelength fluorescence of 5-FAM is also less interfered by the autofluorescence of components in biological samples and test compounds. This assay can detect as low as 0.8 ng/mL active IDE.		카트담기 견적문의
	AS-72223	SensoLyte ® 520 Neprilysin Activity Assay Kit *Fluorimetric*	1 kit	Anaspec	The SensoLyte® 520 Neprilysin Assay Kit employs a novel internally quenched 5-FAM/QXL® FRET substrate for the detection of neprilysin activity. The enzyme cleaves the FRET substrate into two separate fragments resulting in the release of 5-FAM fluorescence, which can be monitored at excitation /emission= 490/520 nm. The long wavelength fluorescence of 5-FAM is less interfered by the autofluorescence of components in biological samples and test compounds. The assay can detect as low as 0.78 ng/mL of active Neprilysin.		카트담기 견적문의
	AS-72197	SensoLyte ® AMC DPPIV Assay Kit *Fluorimetric*	1 kit	Anaspec	The SensoLyte® AMC DPPIV Assay Kit provides a convenient method for high throughput screening (HTS) of DPPIV inhibitors and for continuous assay of DPPIV activity using a fluorogenic substrate that has a high reactivity and low background. DPPIV cleaves the substrate resulting in release of AMC (7-amino-4-methylcoumarin) fluorophore. Fluorescence can be monitored at excitation /emission= 354/442 nm. This kit can be used for high throughput screening of DPPIV inhibitors.		카트담기 견적문의
	AS-72196	SensoLyte ® Rh110 DPPIV Assay Kit *Fluorimetric*	1 kit	Anaspec	The SensoLyte® Rh110 DPPIV Assay Kit provides a convenient method for high throughput screening of DPPIV inhibitors and for continuous assay of DPPIV activity utilizing a fluorogenic peptide. Upon enzyme cleavage, the peptide releases the Rh110 fluorophore with bright green fluorescence that can be detected at Ex/Em=490/520 nm. The longer-wavelength spectra and higher extinction coefficient of the Rh110 provide greater sensitivity and less interference from reaction components.		카트담기 견적문의
	AS-72210	SensoLyte ® Rh110 Enterokinase Activity Assay Kit *Fluorimetric*	1 kit	Anaspec	The SensoLyte® Rh110 Enterokinase Assay Kit is optimized for detection of enterokinase activity using a fluorogenic substrate. Upon enzyme cleavage, this substrate generates the Rh110 (rhodamine 110) fluorophore with bright green fluorescence that can be detected at excitation/emission=490/520 nm. The longer-wavelength spectra and higher extinction coefficient of the Rh110 provide greater sensitivity and less interference from other reaction components. The assay can detect as low as 0.31 ng/mL active enterokinase. Kit size: 100 assays		카트담기 견적문의

	전체선택
	SensoLyte ® 520 Enterokinase Activity Assay Kit *Fluorimetric* Cat No. AS-72209 규격 1 kit 제조사 Anaspec 제품정보 The SensoLyte® 520 Enterokinase Assay Kit is optimized for detecting activity of enterokinase. This kit employs a novel internally quenched 5-FAM/QXL FRET substrate for enterokinase. Enterokinase cleaves the FRET substrate into two separate fragments resulting in release of 5-FAM fluorescence which can be monitored at excitation/emission= 490/520 nm. The long wavelength fluorescence of 5-FAM is less interfered by the autofluorescence of components in biological samples and test compounds. The assay can detect as low as 1.25 ng/mL of active enterokinase. Kit size: 100 assays 수량 카트담기 견적문의
	SensoLyte ® 520 IDE Activity Assay Kit *Fluorimetric* Cat No. AS-72231 규격 1 kit 제조사 Anaspec 제품정보 The SensoLyte® 520 IDE Activity Assay Kit can be used to detect enzyme activity in biological samples or in purified enzyme preparations. The unique FRET substrate was derived from an APP sequence designed to reduce cross reactivity with Neprilysin, ADAM10, TACE, BACE-1, and BACE-2. When active IDE cleaves the FRET substrate, it results in an increase of 5-FAM fluorescence, which is monitored at excitation/emission = 490 nm/520 nm. The long wavelength fluorescence of 5-FAM is also less interfered by the autofluorescence of components in biological samples and test compounds. This assay can detect as low as 0.8 ng/mL active IDE. 수량 카트담기 견적문의
	SensoLyte ® 520 Neprilysin Activity Assay Kit *Fluorimetric* Cat No. AS-72223 규격 1 kit 제조사 Anaspec 제품정보 The SensoLyte® 520 Neprilysin Assay Kit employs a novel internally quenched 5-FAM/QXL® FRET substrate for the detection of neprilysin activity. The enzyme cleaves the FRET substrate into two separate fragments resulting in the release of 5-FAM fluorescence, which can be monitored at excitation /emission= 490/520 nm. The long wavelength fluorescence of 5-FAM is less interfered by the autofluorescence of components in biological samples and test compounds. The assay can detect as low as 0.78 ng/mL of active Neprilysin. 수량 카트담기 견적문의
	SensoLyte ® AMC DPPIV Assay Kit *Fluorimetric* Cat No. AS-72197 규격 1 kit 제조사 Anaspec 제품정보 The SensoLyte® AMC DPPIV Assay Kit provides a convenient method for high throughput screening (HTS) of DPPIV inhibitors and for continuous assay of DPPIV activity using a fluorogenic substrate that has a high reactivity and low background. DPPIV cleaves the substrate resulting in release of AMC (7-amino-4-methylcoumarin) fluorophore. Fluorescence can be monitored at excitation /emission= 354/442 nm. This kit can be used for high throughput screening of DPPIV inhibitors. 수량 카트담기 견적문의
	SensoLyte ® Rh110 DPPIV Assay Kit *Fluorimetric* Cat No. AS-72196 규격 1 kit 제조사 Anaspec 제품정보 The SensoLyte® Rh110 DPPIV Assay Kit provides a convenient method for high throughput screening of DPPIV inhibitors and for continuous assay of DPPIV activity utilizing a fluorogenic peptide. Upon enzyme cleavage, the peptide releases the Rh110 fluorophore with bright green fluorescence that can be detected at Ex/Em=490/520 nm. The longer-wavelength spectra and higher extinction coefficient of the Rh110 provide greater sensitivity and less interference from reaction components. 수량 카트담기 견적문의
	SensoLyte ® Rh110 Enterokinase Activity Assay Kit *Fluorimetric* Cat No. AS-72210 규격 1 kit 제조사 Anaspec 제품정보 The SensoLyte® Rh110 Enterokinase Assay Kit is optimized for detection of enterokinase activity using a fluorogenic substrate. Upon enzyme cleavage, this substrate generates the Rh110 (rhodamine 110) fluorophore with bright green fluorescence that can be detected at excitation/emission=490/520 nm. The longer-wavelength spectra and higher extinction coefficient of the Rh110 provide greater sensitivity and less interference from other reaction components. The assay can detect as low as 0.31 ng/mL active enterokinase. Kit size: 100 assays 수량 카트담기 견적문의

관련제품

	Cat No.	품명	규격	제조사	제품정보	수량	견적문의
	AS-60804	Amylin (1 - 37), human	1 mg	Anaspec	This peptide is a major constituent of protein deposits identified in the Islets of Langerhans of patients with noninsulin-dependent diabetes mellitus.		카트담기 견적문의
	AS-60253-1	Amylin (1 - 37), rat, mouse	1 mg	Anaspec	Rat/mouse Amylin differs from the human version in 6 amino acids: H18R, F23L, A25P, I26V, S28P and S29P (first letter is the amino acid of the human sequence). Unlike the human IAPP (Amylin), rat Amylin does not form amyloid fibers.		카트담기 견적문의
	AS-64451-05	Biotin - Amylin (1 - 37), amide, human	0.5 mg	Anaspec	This amidated amylin (1-37) peptide has a biotin conjugated on the N-terminus. Amylin (1-37) or IAPP (Islet Amyloid Polypeptide Precursor) is a major constituent of protein deposits identified in the Islets of Langerhans of patients with noninsulin-dependent diabetes mellitus.		카트담기 견적문의
	AS-61127	C - peptide (57 - 87), human	0.5 mg	Anaspec	C-peptide is cleaved from proinsulin, stored in secretory granules, and eventually released into the bloodstream in amounts equimolar with those of insulin. The measurement of the C-peptide is an important test for the β-cell function. In the red blood cells of type 2 diabetic patients, Na+,K+ ATPase activity is strongly related to blood C-peptide levels. C-peptide signal transduction in human renal tubular cells involves the activation of phospholipase C and PKC-δ and PKC-varepsilon, as well as RhoA, followed by phosphorylation of ERK1/2 and JNK and a parallel activation of Akt. C-peptide shows specific binding to a G-protein-coupled membrane binding site, resulting in Ca2+ influx, activation of mitogen-activated protein kinase signalling pathways and stimulation of Na+, K+ ATPase and endothelial nitric oxide synthase.		카트담기 견적문의
	AS-24464	Exendin 4	1 mg	Anaspec	Exendin-4, an agonist of glucagon-like peptide 1 (GLP-1) receptor, induces release of insulin after food intake. Exendin-4 shares a 53% sequence homology with GLP-1. Derived from Gila monster, Heloderma suspectum, Exendin-4 has a longer half life than GLP-1 in the plasma, thus making it a more potent insulinotropic agent.		카트담기 견적문의
	AS-60279-1	Biotin - Exendin 4	1 mg	Anaspec	This peptide, Exendin-4, has a biotin on the N-terminus. Exendin-4, an agonist of glucagon-like peptide 1 (GLP-1) receptor, induces release of insulin after food intake. Exendin-4 shares a 53% sequence homology with GLP-1. Derived from Gila monster, Heloderma suspectum, Exendin-4 has a longer half life than GLP-1 in the plasma, thus making it a more potent insulinotropic agent.		카트담기 견적문의
	AS-65070	GLP - 1(9 - 36), amide, human	1 mg	Anaspec	GLP-1 (9-36) is the result of the rapid degradation of GLP-1 (7-36)amide, by the enzyme dipeptidyl peptidase IV (DPP-4), which is widely expressed in a number of sites, including the endothelial cells of small gut arterioles. GLP-1 (9-36) accounts for the majority of GLP-1 that reaches the system circulation. Whereas GLP-1 (7-36) amide stimulates glucose-dependent insulin secretion and inhibits glucagon secretion, GLP-1(9-36)amide administration had no effect on glucose clearance or insulin secretion in humans. GLP-1(9-36)amide however was shown to exert cardioprotective actions in rodent hearts.		카트담기 견적문의
	AS-61226-05	GIP (1 - 42), human	0.5 mg	Anaspec	GIP (Glucose-dependent Insulinotropic Polypeptide or also known as Gastric Inhibitory Polypeptide) is a 42-amino acid peptide released by the K cells of the duodenum and jejunum in response to food intake. GIP, together with GLP (Gastric-like Peptide) are members of the hormone peptide family of Incretins which stimulate insulin secretion from pancreatic islet β-cells, and also appears to promote beta cell proliferation and beta cell survival. Recent studies suggest that GIP plays a role in lipid homeostasis and possibly in the pathogenesis of obesity.		카트담기 견적문의
	AS-20272	Insulin Receptor (1142 - 1153), pTyr(1146, 1150, 1151)	1 mg	Anaspec	Peptides are used as insulin receptor tyrosine kinase substrates.		카트담기 견적문의
	AS-65569	Spexin (36 - 49), amidated, human/mouse/rat	1 mg	Anaspec	Spexin is a novel peptide hormone that is derived from prohormone proNPQ and is conserved amongst mammalian species. Spexin (36-49) is post-translationally amidated with a C-terminal amidation processing signal motif (GRR) following Q49 residue of the pro-hormone. Spexin reduces adipocyte uptake of long chain fatty acids and causes weight loss in rodents. Recently it was suggested that this peptide may play a role in lipid and glucose metabolism in patients with type-2 diabetes mellitus.		카트담기 견적문의
	AS-24277	Somatostatin 14, human, rat, mouse, pig, chicken, frog	1 mg	Anaspec	Somatostatin [SST, GHIH (growth hormone-inhibiting hormone) or SRIF (somatotropin release-inhibiting factor)] is a cyclic peptide existing in two isoforms and is produced in the pancreas islet, GI tract and the central nervous system. Tetradecapeptide Somatostatin-14 (SRIF-14, the originally identified somatostatin) and the 28-amino acid Somatostatin-28 (SRIF-28) have similar biological activities but differ in their potency. Somatostatins inhibit the release of growth hormone in contrast to Growth Hormone Factor (GRF), which stimulates the release of growth hormone. Somatostatins also inhibit the release of prolactin and thyrotropin, peptide hormones from the pituitary gland and glucagon and insulin from the pancreas. It also controls the secretion of gut hormones and functions as a neurotransmitter.		카트담기 견적문의
	AS-63773	Insulin β Chain Peptide (15 - 23)	1 mg	Anaspec	This is amino acids 15 to 23 fragment of the insulin β chain recognized by islet-associated T cells. This peptide is also known as INS. It was used in diabetes studies to assay the IFN-β and IL-17 production by diabetogenic CD4 or CD8 T cells. This peptide stains the INS-reactive CTL clone, but doesn’t stain the splenic CD8+ T cells from NOD or 8.3-TCR-transgenic NOD mice.		카트담기 견적문의

	전체선택
	Amylin (1 - 37), human Cat No. AS-60804 규격 1 mg 제조사 Anaspec 제품정보 This peptide is a major constituent of protein deposits identified in the Islets of Langerhans of patients with noninsulin-dependent diabetes mellitus. 수량 카트담기 견적문의
	Amylin (1 - 37), rat, mouse Cat No. AS-60253-1 규격 1 mg 제조사 Anaspec 제품정보 Rat/mouse Amylin differs from the human version in 6 amino acids: H18R, F23L, A25P, I26V, S28P and S29P (first letter is the amino acid of the human sequence). Unlike the human IAPP (Amylin), rat Amylin does not form amyloid fibers. 수량 카트담기 견적문의
	Biotin - Amylin (1 - 37), amide, human Cat No. AS-64451-05 규격 0.5 mg 제조사 Anaspec 제품정보 This amidated amylin (1-37) peptide has a biotin conjugated on the N-terminus. Amylin (1-37) or IAPP (Islet Amyloid Polypeptide Precursor) is a major constituent of protein deposits identified in the Islets of Langerhans of patients with noninsulin-dependent diabetes mellitus. 수량 카트담기 견적문의
	C - peptide (57 - 87), human Cat No. AS-61127 규격 0.5 mg 제조사 Anaspec 제품정보 C-peptide is cleaved from proinsulin, stored in secretory granules, and eventually released into the bloodstream in amounts equimolar with those of insulin. The measurement of the C-peptide is an important test for the β-cell function. In the red blood cells of type 2 diabetic patients, Na+,K+ ATPase activity is strongly related to blood C-peptide levels. C-peptide signal transduction in human renal tubular cells involves the activation of phospholipase C and PKC-δ and PKC-varepsilon, as well as RhoA, followed by phosphorylation of ERK1/2 and JNK and a parallel activation of Akt. C-peptide shows specific binding to a G-protein-coupled membrane binding site, resulting in Ca2+ influx, activation of mitogen-activated protein kinase signalling pathways and stimulation of Na+, K+ ATPase and endothelial nitric oxide synthase. 수량 카트담기 견적문의
	Exendin 4 Cat No. AS-24464 규격 1 mg 제조사 Anaspec 제품정보 Exendin-4, an agonist of glucagon-like peptide 1 (GLP-1) receptor, induces release of insulin after food intake. Exendin-4 shares a 53% sequence homology with GLP-1. Derived from Gila monster, Heloderma suspectum, Exendin-4 has a longer half life than GLP-1 in the plasma, thus making it a more potent insulinotropic agent. 수량 카트담기 견적문의
	Biotin - Exendin 4 Cat No. AS-60279-1 규격 1 mg 제조사 Anaspec 제품정보 This peptide, Exendin-4, has a biotin on the N-terminus. Exendin-4, an agonist of glucagon-like peptide 1 (GLP-1) receptor, induces release of insulin after food intake. Exendin-4 shares a 53% sequence homology with GLP-1. Derived from Gila monster, Heloderma suspectum, Exendin-4 has a longer half life than GLP-1 in the plasma, thus making it a more potent insulinotropic agent. 수량 카트담기 견적문의
	GLP - 1(9 - 36), amide, human Cat No. AS-65070 규격 1 mg 제조사 Anaspec 제품정보 GLP-1 (9-36) is the result of the rapid degradation of GLP-1 (7-36)amide, by the enzyme dipeptidyl peptidase IV (DPP-4), which is widely expressed in a number of sites, including the endothelial cells of small gut arterioles. GLP-1 (9-36) accounts for the majority of GLP-1 that reaches the system circulation. Whereas GLP-1 (7-36) amide stimulates glucose-dependent insulin secretion and inhibits glucagon secretion, GLP-1(9-36)amide administration had no effect on glucose clearance or insulin secretion in humans. GLP-1(9-36)amide however was shown to exert cardioprotective actions in rodent hearts. 수량 카트담기 견적문의
	GIP (1 - 42), human Cat No. AS-61226-05 규격 0.5 mg 제조사 Anaspec 제품정보 GIP (Glucose-dependent Insulinotropic Polypeptide or also known as Gastric Inhibitory Polypeptide) is a 42-amino acid peptide released by the K cells of the duodenum and jejunum in response to food intake. GIP, together with GLP (Gastric-like Peptide) are members of the hormone peptide family of Incretins which stimulate insulin secretion from pancreatic islet β-cells, and also appears to promote beta cell proliferation and beta cell survival. Recent studies suggest that GIP plays a role in lipid homeostasis and possibly in the pathogenesis of obesity. 수량 카트담기 견적문의
	Insulin Receptor (1142 - 1153), pTyr(1146, 1150, 1151) Cat No. AS-20272 규격 1 mg 제조사 Anaspec 제품정보 Peptides are used as insulin receptor tyrosine kinase substrates. 수량 카트담기 견적문의
	Spexin (36 - 49), amidated, human/mouse/rat Cat No. AS-65569 규격 1 mg 제조사 Anaspec 제품정보 Spexin is a novel peptide hormone that is derived from prohormone proNPQ and is conserved amongst mammalian species. Spexin (36-49) is post-translationally amidated with a C-terminal amidation processing signal motif (GRR) following Q49 residue of the pro-hormone. Spexin reduces adipocyte uptake of long chain fatty acids and causes weight loss in rodents. Recently it was suggested that this peptide may play a role in lipid and glucose metabolism in patients with type-2 diabetes mellitus. 수량 카트담기 견적문의
	Somatostatin 14, human, rat, mouse, pig, chicken, frog Cat No. AS-24277 규격 1 mg 제조사 Anaspec 제품정보 Somatostatin [SST, GHIH (growth hormone-inhibiting hormone) or SRIF (somatotropin release-inhibiting factor)] is a cyclic peptide existing in two isoforms and is produced in the pancreas islet, GI tract and the central nervous system. Tetradecapeptide Somatostatin-14 (SRIF-14, the originally identified somatostatin) and the 28-amino acid Somatostatin-28 (SRIF-28) have similar biological activities but differ in their potency. Somatostatins inhibit the release of growth hormone in contrast to Growth Hormone Factor (GRF), which stimulates the release of growth hormone. Somatostatins also inhibit the release of prolactin and thyrotropin, peptide hormones from the pituitary gland and glucagon and insulin from the pancreas. It also controls the secretion of gut hormones and functions as a neurotransmitter. 수량 카트담기 견적문의
	Insulin β Chain Peptide (15 - 23) Cat No. AS-63773 규격 1 mg 제조사 Anaspec 제품정보 This is amino acids 15 to 23 fragment of the insulin β chain recognized by islet-associated T cells. This peptide is also known as INS. It was used in diabetes studies to assay the IFN-β and IL-17 production by diabetogenic CD4 or CD8 T cells. This peptide stains the INS-reactive CTL clone, but doesn’t stain the splenic CD8+ T cells from NOD or 8.3-TCR-transgenic NOD mice. 수량 카트담기 견적문의

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