제품특징
1 step protocol: 최소 14일 소요
EASY TO USE
- 2D Monolayer culture 방식: 기존 suspension 상태의 세포보다 배양 방법 간편
- EB formation 전용 제품 Aggrewell™ 이용, 균일한 CD34+ HSC EB 배양 가능
HIGH YIELD
- hPSC에서 CD14+ Monocyte의 분화율은 평균 60% 수준
- 6 well plate 1 plate 기준으로 최대 7.1 x 106 cells Monocyte 획득 가능
The average frequency of viable CD14+ monocytes at the peak harvest was 61 - 78%. The average yield of CD14+ monocytes produced per 6-well plate at the peak harvest was between 1.6 x 106 and 7.1 x 106 cells. Data are shown as mean ± SEM (n = 3 - 14)
DOWNSTREAM APPLICATION
- Macrophage research
CD14+ Monocyte로 21일 분화 후 ImmunoCult™-SF Macrophage Medium (ST10961)을 이용하여 Macrophage 분화 배양 가능. Primary M2a Macrophage와 STEMdiff™ Monocyte kit + ImmunoCult™-SF Macrophage Medium 를 사용하여 분화한 PSC-Derived M2a Macrophage의 Phagocytic activity가 동등하게 나타난 것 확인
- Dendritic cell research
CD14+ Monocyte로 21일 분화 후 ImmunoCult™ Dendritic Cell Culture Kit (ST10985)을 이용하여 Dendritic cell (DC) 분화 배양 가능
(A) DC분화 7일 후 CD14⁻CD83⁻/lo immature dendritic cells 확인
(B) DC분화 7일 후 2일간 DC activation 진행. CD14+CD83+ mature dendritic cell 확인
제품사양
| Formulation |
Serum-free |
| Components |
1.STEMdiff™ Hematopoietic Basal Medium, 120 mL 2.STEMdiff™ Hematopoietic Supplement A (200X), 225 μL 3.STEMdiff™ Hematopoietic Supplement B (200X), 225 μL 4.STEMdiff™ Monocyte Differentiation Supplement (100X), 3 x 1 mL 5.StemSpan™ SFEM II, 3 x 100 mL |
| Cell Type |
Dendritic Cells; Macrophages; Monocytes; Myeloid Cells; Pluripotent Stem Cells |
| Species |
Human |
| Application |
Cell Culture; Differentiation; Expansion |
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