제품특징
2 step protocol: 40일 소요
- Step1. CD34+ Hematopoietic stemcell (HSC) EB (~Day12)
- Step2. NK cell Generation (Day12~Day40)
CONSISTENT & UNIFORM
- Serum-free 배지 성분과 Feeder-free 방식 사용
: 동물 유래 성분이나 stroma cell 유래 오염 제거 가능
- EB formation 전용 제품 Aggrewell™ 이용, 균일한 CD34+ HSC EB 배양 가능
HIGH YIELD
- hPSC에서 CD34+ cell 분화율은 최대 42%까지 나타남
- 실제 Aggrewell™ 6 well plate 1 well 기준으로 최대 7.3x105 CD34+ cells 획득 가능
The average frequency of viable CD34+ cells on day 12 for two ES cell lines (H1 and H9) and three iPS cell lines (WLS-1C, STiPS-M001, and STiPS-F016) ranged between 31% and 42%. The average yield of CD34+ cells produced per well of a 6-well AggreWell™400 plate ranged between 3.3 x 105 and 7.3 x 105. Data are shown
- CD34+ cell로부터 CD56+ NK cell의 분화 효율은 평균 80% 이상
- Input CD34+cell 대비 최대 300배 가까운 NK cell 획득 가능
제품사양
| Formulation |
Serum-free |
| Components |
1.STEMdiff™ Hematopoietic - EB Basal Medium, 120 mL 2.STEMdiff™ Hematopoietic - EB Supplement A, 265 μL 3.STEMdiff™ Hematopoietic - EB Supplement B, 7 mL 4.StemSpan™ SFEM II, 2 x 100 mL (Catalog #09605) 5.StemSpan™ Lymphoid Progenitor Expansion Supplement (10X), 5 mL (Catalog #09915) 6.StemSpan™ Lymphoid Differentiation Coating Material (100X), 2 x 0.25 mL (Catalog #09925) 7. StemSpan™ NK Cell Differentiation Supplement (100X), 0.5 mL (Catalog #09950) |
| Cell Type |
Hematopoietic Cells, PSC-Derived; NK Cells; Pluripotent Stem Cells |
| Species |
Human |
| Application |
Cell Culture; Differentiation; Expansion |
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