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Protocol Overview

- Phase (1) : HBEC/HSAEC 1D Expansion Culture: PneumaCult™-Ex Plus 

- Phase (2) : HBEC/HSAEC Air Liquid Interface Expansion Culture: PneumaCult™-Ex Plus 

- Phase (3) : HBEC/HSAEC Air Liquid Interface Maintenance Culture: PneumaCult™-ALI-S or ALI Medium

Fast Cell Expansion & Representative morphology 

같은 배양 조건으로 타 제품과 비교했을 때 

PneumaCult™-Ex Plus 에서 배양한 세포의doubling population이 높고, 작고 촘촘한 morphology를 보임
 

Figure 1. (위) Commercially available, cryopreserved P1 HBECs were seeded into PneumaCult™-Ex Plus, PneumaCult™-Ex, or Bronchial Epithelial Growth Media. Cells cultured in PneumaCult™-Ex Plus have a significantly higher proliferation rate over 9 passages compared to those maintained in either control medium (n=6).

Figure 2. (아래) Representative live culture images for P4 HBECs cultured in PneumaCult™-Ex Plus, PneumaCult™-Ex, or Bronchial Epithelial Growth Media. All images were taken using a 10X objective.

Widespread expression marker (CD49f, CD271) 

PneumaCult™-Ex Plus에서 배양한 HBEC에서 basal cell의 특이적 발현 단백질인 CD49f 과CD271이 높은 효율로 발현됨

Figure 3: Immunocytochemistry 

Figure 4: Flow cytometry  

Figure 3. Immunocytochemistry detection of basal cell markers - CD49f (A, B, and C) and CD271 (D, E, and F) - for P4 HBECs cultured in PneumaCult™-Ex Plus (A and D), PneumaCult™-Ex (B and E), and Bronchial Epithelial Growth Media (C and F). All images were taken using a 10X objective.

Figure 4. P4 HBECs cultured in PneumaCult™-Ex Plus (A), PneumaCult™-Ex (B), and Bronchial Epithelial Growth Media (C) were characterized by flow cytometry to detect expression of the basal cell markers CD49f and CD271. HBECs cultured in PneumaCult™-Ex Plus (A) have a higher proportion of cells coexpressing CD49f and CD271, compared to those cultured in PneumaCult™-Ex (B) and Bronchial Epithelial Growth Media (C).

Airway Epithelial Cell 성격을 지속적으로 유지  

PneumaCult™-Ex Plus에서 배양한 HBEC는 지속적인 maintenance를 진행해도
Later passage에서 Air Liquid Interface (ALI) Epithelial culture시 goblet cell의 분화능을 보임 

Transepithelial electrical resistance (TEER) 반응성 

PneumaCult™-Ex Plus에서 배양한 HBEC 는 여러 가지 Channel activator에 반응을 극대화하여 Ion channel activity 연구에 적합함 

Figure5. Transepithelial electrical resistance (TEER) (A) and representative characterization of the ion channel activities (B) for ALI cultures at 28 days post air-lift using HBECs expanded in PneumaCult™-Ex Plus, PneumaCult™-Ex, or Bronchial Epithelial Growth Media. Amiloride: ENaC inhibitor. IBMX and Forskolin: CFTR activators. Genistein: CFTR potentiator. CFTRinh-172: CFTR inhibitor. UTP: Calciumactivated Chloride channels (CaCCs) activator. All ALI differentiation cultures were performed using PneumaCult™-ALI.

주문정보

	Cat No.	품명	규격	제조사	제품정보	수량	견적문의
	ST05040	PneumaCult™-Ex Plus	1 kit	StemCell Technologies	Serum- and BPE-free medium for expansion of primary human bronchial and small airway epithelial cells (HBEC and HSAEC)		카트담기 견적문의

	전체선택
	PneumaCult™-Ex Plus Cat No. ST05040 규격 1 kit 제조사 StemCell Technologies 제품정보 Serum- and BPE-free medium for expansion of primary human bronchial and small airway epithelial cells (HBEC and HSAEC) 수량 카트담기 견적문의

관련제품

	Cat No.	품명	규격	제조사	제품정보	수량	견적문의
	ST05001	PneumaCult™-ALI Medium	1 kit	StemCell Technologies	Human small airway epithelial cells (HSAEC) 을 in vivo와 유사한 ALI 배양이 가능한 Serum- and BPE-free 배지		카트담기 견적문의
	ST05050	PneumaCult™-ALI-S Medium	1 kit	StemCell Technologies	Human Airway Epithelial Cells (or Human bronchial airway epithelial cell) 을 in vivo와 유사한 ALI 배양이 가능한 Serum- and BPE-free 배지		카트담기 견적문의

	전체선택
	PneumaCult™-ALI Medium Cat No. ST05001 규격 1 kit 제조사 StemCell Technologies 제품정보 Human small airway epithelial cells (HSAEC) 을 in vivo와 유사한 ALI 배양이 가능한 Serum- and BPE-free 배지 수량 카트담기 견적문의
	PneumaCult™-ALI-S Medium Cat No. ST05050 규격 1 kit 제조사 StemCell Technologies 제품정보 Human Airway Epithelial Cells (or Human bronchial airway epithelial cell) 을 in vivo와 유사한 ALI 배양이 가능한 Serum- and BPE-free 배지 수량 카트담기 견적문의

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