제품특징

STEMdiff™ Dorsal Forebrain Organoid Differentiation Kit는 matrix embedding 없이 hPSC로부터 3D의 패턴화된 brain organoid의 생성을 위한 제품입니다. 이 제품은 오가노이드의 융합을 방지하기 위해 AggreWellTM과 함께 사용하며, 키트장 500개 이상의 오가노이드 생성 가능한 무혈청 (Serum-free) 배양 배지 입니다. 

Advantages

- 무융합 (fusion-free) 성장을 위한 시스템으로 media 낭비 감소

- 계통과 개별 오가노이드 간의 높은 재현성

- AggreWellTM800과 함께 사용으로 키트당 500개 이상의 오가노이드 생성

- Matrix가 없는 프로토콜의 사용

- 신경 독성 (neurotoxicity) 및 신경 퇴행 (neurodegenerative) 모델에 대한 장기 배양 및 감소된 caspase-3 발현 

- 질병 모델링 및 재생 응용 application을 위한 assembloids 가능

General Workflow

Figure 1. Schematic for the STEMdiff™ Dorsal Forebrain Organoid Differentiation Kit

Human ES or iPS cell-derived dorsal forebrain organoids can be generated in 43 days. Embryoid bodies can be created in 6 days with AggreWell™800 plates. The EBs are then cultured in suspension, allowing growth and subsequent patterning to the dorsal forebrain. For the long-term maintenance and further maturation of dorsal forebrain organoids, see the PIS.

균질한 오가노이드 생성

Figure 2. STEMdiff™ Dorsal Forebrain Organoid Differentiation Kit Supports the Generation of Homogeneous, Reproducible Neural Organoids

(A) Neural aggregates formed in AggreWell™800 microwell plates exhibit uniform size and shape at day 6. H9-derived dorsal forebrain organoids from a single batch have spherical morphology at days (B) 15 (C) 25 (D) 50 (E) 75 and (F) 100. Scale bar = 1 mm. (G) Dorsal forebrain organoids exhibit homogeneous size over multiple cell lines (average ± SD, using 11 cell lines with 3 - 5 organoids counted per cell line and time point). (H-N) Day 25 organoids generated by a novice user with STEMdiff™ Dorsal Forebrain Organoid Differentiation Kit show reproducibility across different cell lines.

원치 않는 오가노이드의 융합 감소

Figure 3. STEMdiff™ Dorsal and Ventral Forebrain Organoid Differentiation Kits Reduce Unwanted Organoid Fusion

Whole-well images of dorsal forebrain organoid cultures derived from the H9 cell line in STEMdiff™ Forebrain Organoid Expansion Medium (bottom row) vs. the control published formulation (top row) show the extent of fusion typical in control media without orbital shaking. At Day 10 post-seeding in the control formulation, 12/12 organoids that were seeded are developing, but by Day 20 a single, larger organoid can be seen in the well. In STEMdiff™ Forebrain Organoid Expansion Medium, 20/20 seeded organoids are developing and 20/20 are still developing at Day 20 without shaking. While organoids in both culture conditions grow larger between 10 and 20 days, the reduction in organoids and enlarged size in the control formulation suggest a reduction in organoid yield due to organoid fusion.

뇌 영역의 특이적 마커 발현

Figure 4. STEMdiff™ Dorsal Forebrain Organoids Exhibit Cortical Layering and Brain-Region-Specific Marker Expression as They Mature

(A) Day 25 dorsal forebrain organoids display multiple cortical-like regions marked by radialized PAX6+ cells surrounded by MAP2 neurons. (B) Day 50 dorsal forebrain organoids continue to display multiple cortical-like regions marked by PAX6 (green) and MAP2 (magenta). (C) Dorsal forebrain organoids cultured for 100 - 200 days show increasing separation of deep-layer neurons (CTIP2, green; TBR1, cyan) from upper-layer neurons (SATB2, magenta).

뇌 영역의 핵심 마커들의 확인

Figure 5. Neural Organoids Generated with STEMdiff™ Dorsal and Ventral Forebrain Organoid Kits Express Key Markers of Brain-Region-Specific Patterning

RNA from single organoids was harvested at a series of time points and subsequently assayed using bulk RNA-seq (1 data column = 1 organoid). (A) Heat map of select genes shows that dorsal forebrain organoids (DFO) express increasing levels of cortex- and glutaminergic neuron-specific genes from day 25 to 75. (B) Day 25 ventral forebrain organoids (VFO) exhibit high expression of markers of the medial ganglionic eminence and of GABAergic neurons. Heat map scale quantifies gene expression across each row with a normalized z-score for each gene.

주문정보

	Cat No.	품명	규격	제조사	제품정보	수량	견적문의
	ST08620	STEMdiff™ Dorsal Forebrain Organoid Differentiation Kit	1 kit	StemCell Technologies	Cell culture medium kit for robust generation of dorsal forebrain neural organoids from human pluripotent stem cells		카트담기 견적문의

	전체선택
	STEMdiff™ Dorsal Forebrain Organoid Differentiation Kit Cat No. ST08620 규격 1 kit 제조사 StemCell Technologies 제품정보 Cell culture medium kit for robust generation of dorsal forebrain neural organoids from human pluripotent stem cells 수량 카트담기 견적문의

관련제품

	Cat No.	품명	규격	제조사	제품정보	수량	견적문의
	ST07010	Anti-Adherence Rinsing Solution	100mL	STEMCELL Technologies	Rinsing solution for cultureware to prevent cell adhesion		카트담기 견적문의
	ST34811	AggreWell™800	1 Plate (24-well plate)	STEMCELL Technologies	Microwell culture plates for easy and reproducible production of embryoid bodies and spheroids		카트담기 견적문의
	ST100-0120	STEMdiffl™ Neural Organoid Maintenance Kit	1 kit	StemCell Technologies	Cell culture medium kit for long-term maintenance and maturation of patterned neural organoids		카트담기 견적문의

	전체선택
	Anti-Adherence Rinsing Solution Cat No. ST07010 규격 100mL 제조사 STEMCELL Technologies 제품정보 Rinsing solution for cultureware to prevent cell adhesion 수량 카트담기 견적문의
	AggreWell™800 Cat No. ST34811 규격 1 Plate (24-well plate) 제조사 STEMCELL Technologies 제품정보 Microwell culture plates for easy and reproducible production of embryoid bodies and spheroids 수량 카트담기 견적문의
	STEMdiffl™ Neural Organoid Maintenance Kit Cat No. ST100-0120 규격 1 kit 제조사 StemCell Technologies 제품정보 Cell culture medium kit for long-term maintenance and maturation of patterned neural organoids 수량 카트담기 견적문의

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