Comparison of different isolation methods on EV yields by both volume of input serum (per mL, A) 

and amount of input serum protein (per mg as measured by fluorometric Qubit protein assay, B).

(A) A coomassie blue-stained protein gel comparing the protein content of exosome preps isolated using different  

methods shows only a few, defined protein bands in the ExoQuick ULTRA lane compared to the other methods. 

(B) Western blotting of the gel shows that the ExoQuick ULTRA prep contains the highest levels of exosome-specific
markers CD9, CD81, and Hsp70 and the lowest levels of the carryover proteins albumin and IgGH.